Antihyperglycemic and Glycogenesis Effects of Different Fractions of Brassica Oleracea in Alloxan Induced Diabetic Rats

The present work carried out the study to investigate the antihyperglycemic, oral glucose tolerance test (OGTT) and glycogenesis effects of the different fractions (Petroleum ether, ethyl acetate and chloroform) of ethanolic extract of Brassica oleracea. The different fractions of the extract were administered intraperitoneally as a single dose of 150 mg/kg body weight to alloxan induced as well as glucose induced diabetic rats and found to reduce blood glucose level significantly (p<0.05). The different fractions of Brassica oleracea to the alloxaninduced diabetic rats resulted in the significant elevation of liver glycogen content which was decreased by 49% in diabetic control. The plant fractions also improve the glucose tolerance in the glucose induced rats. The effects of plant fractions were compared with standard drug metformin. The phytochemical screening tests indicated that the different constituents such as saponins, tannins, triterpenes, alkaloids and flavonoids etc. were present in the plant which has antidiabetic and glycogenesis properties. Thus, this investigation paves the way for plant based diabetic treatment and indicates that various fractions (Petroleum ether, ethyl acetate and chloroform) of the methanolic extract of Brassica oleracea have favorable effect in bringing down the severity of diabetes, enhancing glycogenesis activity by increasing the cellular uptake of glucose and also improving glucose tolerance activity. INTRODUCTION: Diabetes mellitus is a metabolic disorder in the endocrine system. This dreadful disease is found in all parts of the world and is becoming a serious threat to mankind health. Diabetes is a chronic disorder in metabolism of carbohydrates, proteins, and fat due to absolute or relative deficiency of insulin secretion with/without varying degree of insulin resistance. According to World Health Organization projections, the prevalence of diabetes is likely to increase by 35% by the year 2025 . Recently, the treatment of diabetes mainly involves a sustained reduction in hyperglycemia by the use of biguanides, thiazolidinediones, sulfonylureas Dphenylalanine and α-glucosidase inhibitors in addition to insulin. However, due to unwanted side effects the efficacies of these compounds are debatable and there is a demand for new compounds for the treatment of diabetes 2, . Hence plants have been suggested as a rich, as yet unexplored source of potentially useful antidiabetic drugs. Many traditional plants treatment for diabetes are used throughout the World.